Virus-induced gene silencing, geminivirus resistance
I am interested in virus-induced gene silencing and in geminivirus-host interactions. Geminiviruses, which are DNA viruses that transcribe their genes in plant nuclei, interact in unique ways with plant gene silencing machinery. In collaboration with Drs. Trino Ascensio-Ibanez, Maria Reyes, and Linda Hanley-Bowdoin, we are currently mapping an Arabidopsis locus that confers resistance to viruses in two different genera of the Geminiviridae.
Selected Publications (out of 54)
Hanley-Bowdoin, L., Bejarano, E.R., Robertson, D., Mansoor, S. (2013) Geminiviruses: Masters at redirecting and reprogramming plant processes. Nature Reviews: Microbiology. 11, 777-788.
Robertson, D. (2013) Modulating plant calcium for better nutrition and stress tolerance. ISRN Botany 2013:1-22. (Invited review) http://dx.doi.org/10.1155/2013/952043
Tuttle, J.R., Haigler, C.H., Robertson, D. (2012) Method: Low-cost delivery of the Cotton leaf crumple virus-induced gene silencing system. Plant Methods 8:27-35.
Tuttle. J.R., Idris, A.M., Brown, J.K., Haigler, C.H., Robertson, D. (2008) Geminivirus-mediated Gene Silencing from Cotton Leaf Crumple Virus Is Enhanced by Low Temperature in Gossypium hirsutum. Plant Physiol 148: 41-50.
Jordan, C.V., Shen, W., Hanley-Bowdoin, L., and Robertson, D. (2007) Geminivirus-induced gene silencing of the tobacco retinoblastoma-related gene results in cell death and altered development. Plant Molecular Biology. 65: 163-175.
Smith, C.A., Woloshuk, C.P., Robertson, D., and Payne, G.S. (2007) Silencing of the Aflatoxin Gene Cluster in a Diploid Strain of Aspergillus flavus is Suppressed by Ectopic aflR Expression. Genetics. 176:2077-2086.
Blevins, T., Rajeswaran, R., Shivaprasad, P.V., Beknazariants, D., Si-Ammour, A., Park, H.-S. Vazquez, F. Robertson, D., Meins, F., Hohn, T. and Pooggin, P., 2006 Four plant Dicers mediate viral small RNA biogenesis and DNA virus induced silencing. Nucleic Acids Research 34:6233-6246.
Muangsan, N., Beclin, C., Vaucheret, H., Robertson, D. (2004) Geminivirus VIGS of endogenous genes requires SGS2/SDE1 and SGS3 and defines a new branch in the genetic pathway for silencing in plants. Plant J. 38: 1004-1014.
Robertson, D. (2004) VIGS vectors for gene silencing, many targets, many tools. Ann. Rev. Plant Biol. 55: 495-519.
Nagar, S., Hanley-Bowdoin, L., and Robertson, D. (2002) Host DNA Synthesis is Induced by Geminivirus Infection of Differentiated Plant Cells. The Plant Cell. 14:2995-3007.
Turnage, M.A., Muangsan, N., Peele, C.G, and Robertson, D. (2002) Geminivirus-based vector for gene silencing in Arabidopsis. Plant J. 30: 107-114.
Peele, C., Jordan, C.V., Muangsan, N., Turnage, M., Egelkrout, E., Eagle, P., Hanley-Bowdoin, L., and Robertson, D. (2001) Silencing of a meristematic gene using geminivirus-derived vectors. Plant J. 27:357-66.
Bass, H., Nagar, S., Hanley-Bowdoin, L. and Robertson, D. (2000) Chromosome condensation induced by geminivirus infection of mature plant cells. J. Cell Sci. 113: 1149-1160.
Kjemtrup, S., Sampson, K.S., Peele, C., Nguyen, L.V., Conkling, M.A., Thompson, W.F. and Robertson, D. (1998) Gene silencing from plant DNA carried by a geminivirus. The Plant Journal, 14: 91-100.
Nagar, S. Pedersen, T., Carrick, K., Hanley-Bowdoin, L. and Robertson, D. (1995) A geminivirus induces expression of a host DNA synthesis protein in terminally differentiated plant cells. The Plant Cell 7: 705-719.