Dr. José M. Bruno-Bárcena

Associate Professor of Microbiology

Bioprocessing and Fermentation, Gram positive organisms

Research Interests

There is a critical need for the systematic identification and testing of new gene modulated states that participate in the enhancement of cellular stress resistance. In our laboratory we use the new era of genomic tools that make it possible to identify point mutations, insertions, deletions and/or translocations that have occurred in offspring strains following defined selection processes. In the near future, this comparative approach will provide valuable insights for identifying gene combinations and biochemical pathways that play a role in microbial metabolism and this information may be applied to further improve production using strategic strain modifications. Our primary research interests are the study of Gram positive organisms and we investigate different, but complementary, areas of study with a focus on functional probiosis and bioenergy. Thus far our bioprocessing research has generated technology, peer-reviewed publications, and intellectual property to be directly implemented and translated into the generation of biofuels and functional foods. We have dedicated significant amounts of time towards implementing these new overlapping bioprocessing research areas that target prime university interests.

List of Publications

WJ Sandoval-Espinola, Chinn M, Thon MR, Bruno-Bárcena JM (2017). Evidence of mixotrophic carbon-capture by n-butanol-producer Clostridium beijerinckii. Scientific Reports 7: 12759 |DOI:10.1038 /s41598-017-12962-8.

JM Whitham, Schulte MJ, Bobay BG, Bruno-Bárcena JM, Chinn MS, Flickinger MC, Pawlak JJ, Grunden AM (2017) Characterization of Clostridium ljungdahlii OTA1: a non-autotrophic hyper ethanol-producing strain. Appl Microbiol Biotechnol. doi:10.1007/s00253-016-7978-6

Mera, A, Arthur, JC, Jobin, C, Keku, TO, Bruno Bárcena, JM, and Azcárate-Peril, MA. (2016) Enriched galacto-oligosaccharides (GOS) enhance specific Bifidobacterium species and their metabolic activity in the mouse gut microbiome. Beneficial Microbes. 11;7(2):247-64

Noar JD, Bruno-Bárcena JM (2016) Protons and pleomorphs: aerobic hydrogen production in Azotobacters.  World J Microbiol Biotechnol. Feb;32(2):29. doi: 10.1007/s11274-015-1980-5

Dagher SF, Bruno-Bárcena JM (2015) A novel N-terminus region of the membrane β-hexosyltransferase; its role in secretion of soluble protein by Pichia pastoris. Microbiology. 2015 Nov 9. doi: 10.1099/mic.0.000211.

Sandoval-Espinola WJ, Chinn M, Bruno-Barcena JM (2015) Inoculum optimization of Clostridium beijerinckii for reproducible growth. FEMS Microbiol Lett. Oct;362(19). doi: 10.1093/femsle/fnv164. 

Noar J, Loveless T, Navarro-Herrero JL, Olson JW, Bruno-Bárcena JM (2015) Aerobic hydrogen production via nitrogenase in Azotobacter vinelandii CA6. Appl Environ Microbiol. Jul;81(13):4507-16. doi: 10.1128/AEM.00679-15

Utturkar SM, Klingeman DM, José M Bruno-Barcena JM, Chinn MS, Grunden AM, Köpke M, Brown SD (2015) Sequence data for Clostridium autoethanogenumusing three generations of sequencing technologies. Sci. Data. 2:150014 doi: 10.1038/sdata.2015.14

Bruno-Barcena JM, Azcarate-Peril  MA (2015) Galacto-oligosaccharides and Colorectal Cancer: Feeding our Intestinal Probiome. J Funct Foods 12:92-108

Noar J, Makwana ST, Bruno-Bárcena JM. (2014) Complete Genome Sequence of Solvent-Tolerant Clostridium beijerinckii Strain SA-1. Genome Announc. 2014 Dec 18;2(6). pii: e01310-14. doi: 10.1128/genomeA.01310-14.

Sandoval WJ, Makwana ST, Chinn MS, Thon MR, Azcárate-Peril MA, Bruno-Bárcena JM (2013) Comparative phenotypic analysis and genome sequence of Clostridium beijerinckii SA-1, an offspring of NCIMB 8052.  Microbiology. 159:2558-70

Bruno-Bárcena JM, Chinn MS, Grunden AM (2013) Genome Sequence of the Autotrophic Acetogen Clostridium autoethanogenum JA1-1 Strain DSM 10061, a Producer of Ethanol from Carbon Monoxide. Genome Announc. 2013 Aug 15;1(4). doi:pii: e00628-13. 10.1128/genomeA.00628-13.

Noar JD, Bruno-Bárcena JM (2013) Complete genome sequences of Azotobacter vinelandii wild-type strain CA and tungsten-tolerant mutant strain CA6. Genome Announc. 1(3):e00313-13. doi:10.1128/genomeA.00313-13.

Dagher SF, Azcarate-Peril MA, Bruno-Bárcena JM (2013) Heterologous expression of a bioactive β-hexosyl-transferase, an enzyme producer of prebiotics, from Sporobolomyces singularis. Appl Environ Microbiol. 79(4):1241-9

Azcarate-Peril MA, Sikes M, and Bruno-Bárcena JM (2011) The intestinal microbiota, gastrointestinal environment and colorectal cancer: A putative role for probiotics in prevention of colorectal cancer. Am J Physiol Gastrointest Liver Physiol. 2011 Sep;301(3):G401-24.

Heluane H, Evans MR, Dagher SF and Bruno-Bárcena JM (2011) Meta-Analysis and Functional Validation of Nutritional Requirements of Solventogenic Clostridia Growing under Butanol Stress Conditions and Coutilization of D-Glucose and D-Xylose. App. Environ. Microbiol. 77(13):4473-85.

Bruno-Bárcena JM, Azcárate-Peril MA, and Hassan HM (2010) Role of antioxidant enzymes in bacterial resistance to organic acids. Appl. Environ. Microbiol. 76: 2747-2753

Dagher SF, Ragout AL, Siñeriz F, Bruno-Bárcena JM (2010) Cell Immobilization for Production of Lactic Acid: Biofilms do it naturally. Advances in Applied Microbiology – Volume 71 / ISBN 978-0-12-3809933

Carroll IM, Andrus JM, Bruno-Barcena JM, Klaenhammer TR, Hassan HM, Threadgill DS (2007) The Anti-inflammatory Properties of Lactobacillus gasseri Expressing Manganese Superoxide Dismutase (MnSOD) Using the Interleukin 10-Deficient Mouse Model of Colitis. Am J Physiol Gastrointest Liver Physiol. 293:G729-38.

Pico-Marco E, Navarro-Herrero JL, Bruno-Bárcena JM (2006) A closed loop exponential feeding law: invariance and global stability analysis. J. Process Control. 16:395-402

Azcárate-Peril MA, Bruno-Bárcena JM, Hassan HM, Klaenhammer TR (2006) Transcriptional Analysis of Oxalyl-CoA Decarboxylase and Formyl-CoA Transferase Genes from Lactobacillus acidophilus. Appl. Environ. Microbiol. 72: 1891-1899

Pérez-Torrado R, Bruno-Bárcena JM, Matallana E (2005) Monitoring stress related genes during the process of biomass propagation of Saccharomyces cerevisiae strains used for wine making. Appl. Environ. Microbiol. 71: 6831-6837

Bruno-Bárcena JM, Azcárate-Peril MA, Klaenhammer TR, Hassan HM (2005) Marker-free Chromosomal Integration of the Manganese Superoxide Dismutase Gene (sodA) from Streptococcus thermophilus into Lactobacillus gasseri. FEMS Microbiol. Lett. 246: 91-101

J.M. Bruno-Bárcena, J.M. Andrus, S. Libby, T.R. Klaenhammer and H.M. Hassan (2004) Expression of a Heterologous Manganese Superoxide Dismutase Gene in Intestinal lactobacilli provides protection against hydrogen peroxide toxicity. Appl. Environ. Microbiol. 70: 4702-4710

J.M. Bruno-Bárcena, M.E. Lucca, F. Siñeriz and D. Ramón (2002) pH regulation of enzyme production in Aspergillus nidulans growing in aerobic carbon-limited batch fermentor. Biotechnol. Lett. 24: 567-572

G. Raya Tonetti, P. R. Córdoba, J.M. Bruno-Bárcena, F. Siñeríz and N. I. Perotti (1999) Fluidized bed ion exchange for improving recovery of lactic acid from fermentation. Biotechnol. Tech. 13: 201-205

J.M. Bruno-Bárcena, A.L. Ragout, P.R. Córdoba and F. Siñeríz (1999) Continuous production of L(+) lactic acid by Lactobacillus casei in two-stage systems. Appl. Microbiol. Biotechnol. 51: 316-324

J. M. Bruno-Bárcena, F. Siñeríz, D. González de Llano, A. Rodríguez and J.E. Suárez (1998) Chemostat production of  plantaricin c by Lactobacillus plantarum LL 441. Appl. Environ. Microbiol. 64: 3512-3514

J.M. Bruno-Bárcena, M.A. Azcárate, A.L. Ragout, G. Font de Valdez, R.R. Raya and F. Siñeríz (1998) Fragile cells of Lactobacillus casei suitable for plasmid DNA isolation. Biotechnol. Tech. 12: 97-99

S. C. Ginés, J. M. Bruno–Bárcena, A. Ragout, G. F. de Valdez (1997) Effect of the carbon source on the fermentation balance of Lactobacillus reuteri. Microbiologie-Aliments-Nutrition. 15: 23-27

G.F. de Valdez, A.L. Ragout, J.M. Bruno-Bárcena, H. Diekmann, and F. Sineriz (1997) Shifts in the pH affect the maltose glycerol cofermentation by Lactobacillus reuteri.Biotechnol. Lett. 19: 645-649

Book Chapters:

M.C.T. Leite, B. Troxell, J.M. Bruno-Bárcena, and H.M. Hassan (2014) Biology of Reactive Oxygen Species, Oxidative Stress, and Antioxidants in Lactic Acid Bacteria. In Koen Venema and Ana Paula do Carmo (Eds). “Probiotics and Prebiotics: Current Research and Future Trends”. Caister Academic Press

J.M. Bruno-Bárcena, E. Picó-Marco, J.L. Navarro, and J. Picó (2007) Microbial Dynamic Transformations: Basic Concepts. Encyclopedia of Life Support Systems (EOLLS). UNESCO

J.M. Bruno-Bárcena and F. Siñeríz (2002) Industrial Biotechnology. Encyclopedia of Life Support Systems (EOLLS). UNESCO

J.M. Bruno-Bárcena, M.A. Azcárate-Peril, and F. Siñeríz (2001) Genetically modified organisms (GMO) in food products: potential risks and the reaction of the consumer. Food Protocols, 481-484. Humana press, Totowa-New Jersey.

J.M. Bruno-Bárcena, A.L. Ragout, P.R. Córdoba, and F. Siñeríz (2001) Reactor configuration for fermentation in immobilized continuous system. Food Protocols, 321-328. Humana press, Totowa-New Jersey.

J.M. Bruno-Bárcena, A.L. Ragout, and F. Siñeríz (1998) Microbial physiology applied to process optimisation: lactic acid bacteria. Advances in Bioprocess Engineering II. Vol. II, 97-110. Kluwer Academic Publishers, The Netherlands.